Help | K9CircRNA-HeartDB

Introduction

K9HeartCircDB is a publicly accessible database of circular RNAs (circRNAs) derived from canine left ventricular tissues in a tachypacing-induced model of non-genetic dilated cardiomyopathy. It enables researchers to explore circRNA features, including divergent primers, siRNAs, predicted miRNA binding sites, and novel polypeptides encoded by circRNAs. The platform serves as a translational resource for studying circRNA-mediated mechanisms in cardiac remodeling and heart failure.

Navigating the Home Page

circRNA Search

Enter a gene, circRNA ID, or a keyword in the “Enter Query circRNA” search bar to explore desired circRNA

Interactive Elements

The K9HeartCircDB contains interactive hyperlink elements that link to the Downloads page in a new page. This allows for quick access to raw data (e.g., IRES, OFFs, Orthologs, etc.) for downstream analysis

UCSC Genome Browser and Other Databases

K9HeartCircDB provides links to UCSC Genome Browser along with other related circRNA databases for further analysis and information on selected circRNA of study

Navigating Search Results

Search Results

All results from quick search will be displayed and sorted based on K9db-ID, and include the corresponding ROS_Cfam1_Chr_ID. To get further information about the circRNA, you can click the “Show More” or “Full Details” option

The “Show More” button provides quick statistics on ORF, miRNA, and IRES information on selected circRNA

Full Details

The “Full Detail” button takes you to another page and provides advanced information on the selected circRNA

Information includes:

Genomic Location (e.g., Chromosome, Start/End, Strand)
Sequence (e.g., Exon, Length, Spliced Sequence)
- Spliced Sequence can be directly copied for further analysis
circRNA ID Conversion (e.g., Other Dog Database IDs, Human Ortholog IDs, Mouse Ortholog IDs)
Divergent Primers (e.g., FP1, FP2, RP1, RP2, PCR Template)
siRNAs for circRNA (e.g., BSJ Sequence, siRNA Sequence)

Navigating the Downloads Page

K9HeartCircDB provides raw data on circRNA that can be downloaded as Excel files

Downloads Include:

Internal Ribosome Entry Sites (IRES)
microRNA Target Sites
Annotated Open Reading Frames
Human and Mouse Orthologs
circRNA Differential Expression